Investigation of Copper Binding to Hydrosoluble Proteins of an Amphipod

Autores

  • Grasiela Lopes Leães Pinho Universidade Federal do Rio Grande (FURG), Rio Grande, RS, Brazil
  • R. Saint-Louis Institut des Sciences de la Mer de Rimouski (ISMER), Université du Québec, Rimouski, Quebec, Canada
  • A. Bianchini Universidade Federal do Rio Grande (FURG), Rio Grande, RS, Brazil
  • E. Pelletier Institut des Sciences de la Mer de Rimouski (ISMER), Université du Québec, Rimouski, Quebec, Canada

DOI:

https://doi.org/10.5132/jbse.2009.01.011

Palavras-chave:

acumulação de cobre, cromatografia de exclusão por tamanho, Gammarus sp., ICPMS, metalotioneínas

Resumo

This study investigated the body copper accumulation in the amphipod Gammarus sp.. Groups of copper-binding proteins were also investigated considering their molecular weights. Regarding copper accumulation, a saturation type kinetics was observed with Km and Vmax values of 0.48 mg Cu.L–1 and 713.3 μg Cu.g–1 dw, respectively. Size exclusion chromatography (5 to 150 KDa) associated with ICP-MS showed that most of copper-binding proteins in Gammarus sp. have molecular weights <12 KDa, probably corresponding to metallothioneins.

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Publicado

2009-06-20

Como Citar

Pinho, G. L. L., Saint-Louis, R., Bianchini, A., & Pelletier, E. (2009). Investigation of Copper Binding to Hydrosoluble Proteins of an Amphipod. Ecotoxicology and Environmental Contamination, 4(1), 83–86. https://doi.org/10.5132/jbse.2009.01.011

Edição

v. 4 n. 1 (2009)

Seção

Artigos

Licença

Copyright (c) 2009 Ecotoxicology and Environmental Contamination

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