Investigation of Copper Binding to Hydrosoluble Proteins of an Amphipod

Authors

  • Grasiela Lopes Leães Pinho Universidade Federal do Rio Grande (FURG), Rio Grande, RS, Brazil
  • R. Saint-Louis Institut des Sciences de la Mer de Rimouski (ISMER), Université du Québec, Rimouski, Quebec, Canada
  • A. Bianchini Universidade Federal do Rio Grande (FURG), Rio Grande, RS, Brazil
  • E. Pelletier Institut des Sciences de la Mer de Rimouski (ISMER), Université du Québec, Rimouski, Quebec, Canada

DOI:

https://doi.org/10.5132/jbse.2009.01.011

Keywords:

copper accumulation, Gammarus sp., ICPMS, metallothioneins, size exclusion chromatography

Abstract

This study investigated the body copper accumulation in the amphipod Gammarus sp.. Groups of copper-binding proteins were also investigated considering their molecular weights. Regarding copper accumulation, a saturation type kinetics was observed with Km and Vmax values of 0.48 mg Cu.L–1 and 713.3 μg Cu.g–1 dw, respectively. Size exclusion chromatography (5 to 150 KDa) associated with ICP-MS showed that most of copper-binding proteins in Gammarus sp. have molecular weights <12 KDa, probably corresponding to metallothioneins.

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Published

20-06-2009

How to Cite

Pinho, G. L. L., Saint-Louis, R., Bianchini, A., & Pelletier, E. (2009). Investigation of Copper Binding to Hydrosoluble Proteins of an Amphipod. Ecotoxicology and Environmental Contamination, 4(1), 83–86. https://doi.org/10.5132/jbse.2009.01.011

Issue

Vol. 4 No. 1 (2009)

Section

Original Articles

License

Copyright (c) 2009 Ecotoxicology and Environmental Contamination

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