Behavior of Cubaris murina Brandt (Crustacea: Isopoda) in Soil Contaminated With Glyphosate: Avoidance Tests in Laboratory
DOI:
https://doi.org/10.5132/jbse.2006.01.003Keywords:
ecotoxicology, isopoda, glyphosate, mortality, avoidance testsAbstract
Acute tests and avoidance tests were carried out using laboratory populations of Cubaris murina, to assess their sensibility to glyphosate. The contaminant solutions were prepared by herbicide Roundup®. In the acute tests, ten offspring were confined and exposed to each treatment: soil with different concentrations of glyphosate and soil without contaminant (control). Mortality was observed after 7 days. The Spearman-Karber test was used to calculate the LC50 . In the avoidance tests, ten juveniles were put on the center of each recipient-test, with four portions of soil: three portions with different concentrations of glyphosate and one control. The preference of the isopods was registered after 1.5, 2, 3, 4 and 24 hours. ANOVA was used to verify whether there were significant differences among the mean number of isopods in each treatment and the control. The 7-d-LC50 was 35.5 mg gli/g soil. In the avoidance tests, significant differences (p < 0.05) in relation to the control, which indicated avoidance, occurred in all treatments, including 5.12 mg gli/g soil, already at 1.5 hour. The avoidance behavior was a very sensitive endpoint, assessed in low cost experiments, showing a rapid response and having an important ecological role.
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