Study of Alterations in the Concentration of Ions in the Plasma and of the Induction of Micronucleus in Piaractus mesopotamicus Exposed to the Herbicide Atrazine

Authors

  • Sandro Estevan Moron Universidade Federal do Tocantins (UFT). TO, Brazil
  • V. L. P. Polez Universidade Brasília (UNB). Brasília, DF, Brazil
  • R. F. Artoni Universidade Estadual de Ponta Grossa (UEPG). Ponta Grossa, PR, Brazil
  • J. L. C. Ribas Universidade Estadual de Ponta Grossa (UEPG). Ponta Grossa, PR, Brazil
  • H. K. Takahashi Universidade Oeste Paulista (UNOESTE). SP, Brazil

DOI:

https://doi.org/10.5132/jbse.2006.01.006

Keywords:

micronucleus, herbicide, plasma ions, Piaractus mesopotamicus

Abstract

The pollution of the aquatic ecosystem has been increasing in the last years, mainly due to the industrial and agricultural development. The Atrazine pesticide is an important agent pollutant and it can harm the development and reproduction of several species of fish due to its toxicity. Piaractus mesopotamicus (pacu) is one of the species thoroughly comercialized and used in fish farms. The present study analyzed possible modifications in the concentration of ions in the plasma (Na+, Cl–, K+, Ca2+), in the blood pH and in the micronucleus frequency in pacu erythrocytes (N = 10 for group; Wt: 160.0 ± 5 g) exposed to the Atrazine herbicide (0.1 mg/L) during 96 hours in semi-static system. Samples of blood were collected, with aid of syringes heparinized, being centrifuged later and/or made smears for subsequent analysis of the micronucleus. The obtained plasma was used for the determinations of ions in the plasma. Cytological analyses showed significant differences (p < 0.0001) in the micronucleus frequency among the control group which was exposed to the atrazine herbicide. The blood pH stayed constant, but the ions concentration in the plasma Na+ and Cl– was, in relation to the control, significantly reduced (p < 0.05). The tested concentration of the herbicide probably provoked disturbances in the acid-base ionic balance and it can also be considered a clastogenic and poisonous product.

Downloads

Published

20-06-2006

How to Cite

Moron, S. E., Polez, V. L. P., Artoni, R. F., Ribas, J. L. C., & Takahashi, H. K. (2006). Study of Alterations in the Concentration of Ions in the Plasma and of the Induction of Micronucleus in Piaractus mesopotamicus Exposed to the Herbicide Atrazine. Ecotoxicology and Environmental Contamination, 1(1), 27–30. https://doi.org/10.5132/jbse.2006.01.006

Issue

Vol. 1 No. 1 (2006)

Section

Original Articles

License

Copyright (c) 2006 Ecotoxicology and Environmental Contamination

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright © 2006 ECOTOX-Brasil

Copyright notice: It is a condition for publication that manuscripts submitted to this journal have not yet been published and will not be simultaneously submitted or published elsewhere. By submitting a manuscript, the authors agree that copyright for their article is transferred to the Sociedade Brasileira de Ecotoxicologia (ECOTOX-Brasil) if and when the article is accepted for publication. The copyright covers the exclusive rights to reproduce and distribute articles, including reprints, photographic reproductions or any other reproduction of a similar nature, including translations. No part of this publication may be reproduced, stored in a retrieval system or transmitted in any form or by any means, electronic, mechanical, photocopying, recording or otherwise, without permission of the publisher.

Notice: While every effort is made by the EEC, editors and editorial board to see that no inaccurate or misleading data, opinions or statements appear in this journal, they wish to make it clear that the contents of the articles and advertisements published herein are the sole responsibility of the contributors or advertisers concerned. Accordingly, the EEC, the editorial board and editors and their respective employees, officers and agents accept no responsibility or liability whatsoever for the consequences of any inaccurate or misleading data, opinion or statement.