Use of Freshwater Planarians Dugesia (Girardia) tigrina Testing Toxicity of a Petroleum Refinery Wastewater
DOI:
https://doi.org/10.5132/jbse.2006.01.014Keywords:
chronic toxicity, freshwater planarians, petroleum’s effluent, water, Dugesia (Girardia) tigrinaAbstract
The action of wastewater from Paulínia Petroleum Refinery (REPLAN) was tested in freshwater planarian regeneration, from the species Dugesia (Girardia) tigrina. This chronic toxicity test was realized to establish conditions to use them as a monitoring species of the refinery treatment efficiency. The tested samples wore collected at six points: rio Jaguari (REPLAN water intake); wastewater beginning biological treatment (after physical treatment); wastewater entering stabilization lagoon (last stage of treatment); discharge of stabilization lagoon; upstream from effluent discharge at rio Atibaia; downstream from the effluent discharge. Drinking water was used as control. Five or eight adult planarian at regeneration stage was tested in each point. The adult tested specimens were selected and weren’t feed during the experiment. The test was initiated immediately after the removal of planarian’s head (cut with blade) and finalized when the regeneration was complete. It was detected high toxic effect only at the sample from the primary treatment discharge, before realizing the aeration and biological treatment. We can conclude that REPLAN effluent, at the end of the treatment process don’t cause any effect in Dugesia (Girardia) tigrina cephalic regeneration.
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