Use of the Cell Quota and Chlorophyll Content for Normalization of Cylindropermopsin Produced by Two Cylindrospermopsis raciborskii Strains Grown Under Different Light Intensities

Authors

  • Ronaldo Leal Carneiro Laboratory of Cyanobacterial Ecophysiology and Toxicology, Institute of Biophysics Carlos Chagas Filho, Health Sciences Center, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil
  • Alessandra Pereira Ribeiro da Silva Companhia Estadual de Águas e Esgotos (CEDAE-RJ), Rio de Janeiro, RJ, Brazil
  • Valeria Freitas de Magalhães Laboratory of Cyanobacterial Ecophysiology and Toxicology, Institute of Biophysics Carlos Chagas Filho, Health Sciences Center, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil
  • Sandra Maria Feliciano de Oliveira e Azevedo Laboratory of Cyanobacterial Ecophysiology and Toxicology, Institute of Biophysics Carlos Chagas Filho, Health Sciences Center, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil

DOI:

https://doi.org/10.5132/eec.2013.01.013

Keywords:

Cylindrospermopsis raciborskii, CYN cell quota, cylindrospermopsin per Chlorophyll-a

Abstract

The production of cylindrospermopsin (CYN) by Cylindrospermopsis raciborskii (Woloszynska) Seenaya and Subba Raju (Cyanobacteria) was already shown to be changed by variations in light intensities. Influences of light intensity on CYN production by two different C. raciborskii strains (CYP011K and CYP030A) were studied in batch culture conditions. The strains were grown under photon light intensities from 40 to 348 μmol photons m-2 s-1. The growth rates were measured by exponential regression of cell number variation along the time. The concentration of chlorophyll-a (Chl-a) was measured by spectrophotometric methods and the CYN concentration was measured by high-performance liquid chromatographic methods. Data from these CYN analyses were evaluated as cell quota (ng CYN 10-6 cells) or as the relative proportion between toxin concentration and chlorophyll-a concentration (CYN Chl-a-1). The CYP011K showed higher growth rates than the CYP030A strains. According to the cell quota results, the production of CYN by these two strains of C. raciborskii is not affected by the intensity of the surrounding light during growth. However, analysis of the relative proportion of CYN Chl-a-1 led to different interpretations, as a negative relation between light intensity and CYN production.

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Published

30-11-2013

How to Cite

Carneiro, R. L., Silva, A. P. R. da, Magalhães, V. F. de, & Oliveira e Azevedo, S. M. F. de. (2013). Use of the Cell Quota and Chlorophyll Content for Normalization of Cylindropermopsin Produced by Two Cylindrospermopsis raciborskii Strains Grown Under Different Light Intensities. Ecotoxicology and Environmental Contamination, 8(1), 93–100. https://doi.org/10.5132/eec.2013.01.013

Issue

Vol. 8 No. 1 (2013)

Section

Original Articles

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Copyright (c) 2013 Ecotoxicology and Environmental Contamination

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