The Mutagenic Effect of the Free and Nanoencapsulated Cooper Edetate in Mice and Fish

Authors

  • Ana Cristina Jacacobowski PPG/Biotecnologia, Universidade Católica Dom Bosco (UCDB), Campo Grande, MS, Brazil
  • N. N. Zobiole PPG/Biotecnologia, Universidade Católica Dom Bosco (UCDB), Campo Grande, MS, Brazil
  • P. M. Padilha Instituto de Biociências, Universidade Estadual Paulista (UNESP), Botucatu, SP, Brazil
  • S. E. Moreno PPG/Biotecnologia, Universidade Católica Dom Bosco (UCDB), Campo Grande, MS, Brazil
  • E. J. Arruda Faculdade de Ciências Exatas e Tecnologia/Química, Universidade Federal da Grande Dourados (UFGD), Dourados, MS, Brazil

DOI:

https://doi.org/10.5132/eec.2013.01.002

Keywords:

Copper edetate, Fish, Micronucleus test, Mouse, Mutagenicity, Nanocapsules

Abstract

Recent studies show that copper complexes with ethylenediaminetetraacetic acid ([Cu(EDTA)]-2), is lethal for Aedes aegypti larvae. This compound may have a controlled release when nanocapsules complex in gum/gelatin/chitosan, which may limit their toxicity to the environment. However, the genotoxicity of [Cu(EDTA)]-2 is not fully elucidated. To assess the mutagenic potential of [Cu(EDTA)]-2, we analyzed the frequency of micronuclei in peripheral blood of mice and fish subjected to free and nanoencapsulated [Cu(EDTA)]-2. The carcass of the fish was assessed by mass spectrometry to measure tissue copper. In mice, the frequency of micronuclei in animals exposed to free [Cu(EDTA)]-2 was significantly higher in the animals submitted to the nanoencapsulated form. In fish, only the free [Cu(EDTA)]-2 was able to induce increased frequency of micronuclei. Mass spectrometric analysis showed copper accumulation in the fish’s liver studied, with significant difference in the control. In the gills of fish were not observed differences in copper concentration between the groups and the copper content was significantly lower than that found in the liver. The results suggest that free [Cu(EDTA)]-2 can induce mutagenic effects in mammals, but the nanoencapsulated seems to give more security to the product due to its slower release.

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Published

18-06-2013

How to Cite

Jacacobowski, A. C., Zobiole, N. N., Padilha, P. M., Moreno, S. E., & Arruda, E. J. (2013). The Mutagenic Effect of the Free and Nanoencapsulated Cooper Edetate in Mice and Fish. Ecotoxicology and Environmental Contamination, 8(1), 13–19. https://doi.org/10.5132/eec.2013.01.002

Issue

Vol. 8 No. 1 (2013)

Section

Original Articles

License

Copyright (c) 2013 Ecotoxicology and Environmental Contamination

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