Multixenobiotic Resistance Mechanism Monitoring: Standardization of Fluorescence Emmited by Rhodamine B

Authors

  • Marcos Luiz Pessatti Centro de Ciência Tecnológica da Terra e do Mar (CTTMar), Universidade do Vale do Itajaí (UNIVALI), Itajaí, SC, Brazil ; Departamento de Bioquímica, Universidade Federal do Paraná, Curitiba, PR, Brasil
  • José Domingos Fontana Departamento de Química e Biologia, Universidade Tecnológica Federal do Paraná (UTFPR), Curitiba, PR, Brazil

DOI:

https://doi.org/10.5132/eec.2013.01.014

Keywords:

MXR, P-glycoprotein, fluorescence, P. perna, mussel, rhodamine

Abstract

Many aquatic organisms express the multixenobiotic resistance mechanism (MXR) mediated by a membrane protein denominated P-glycoprotein (Pgp), which reduce the accumulation of xenobiotics by active transport to out of cells. In order to establish fluorescence microscopy as a quantitative method to monitor MXR activity, the kinetic of fluorescence decay emitted by rhodamine B (RB) was determined. Rhodamine B (1 at 1000 nmoles L-1) were spotted on silica gel plate and fluorescence decay recorded and determined as exposition time (ET, sec) using a fluorescence microscope’s photo sensor. The ET at zero time was obtained from a linear equation of plotting ET(s) against the respective rhodamine B concentrations. The resulting mathematical model (RB = (28/ET) -1 -Blank, r2 = 0.9945), allowed the quantitative determination of rhodamine B intracellular accumulation (nmoles L-1This transport activity quantitation is consequence of the MXR mechanism activity operating in viable sections of gills of the mussel Perna perna and allows its measurement as a molecular biomarker.

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Published

19-06-2013

How to Cite

Pessatti, M. L., & Fontana, J. D. (2013). Multixenobiotic Resistance Mechanism Monitoring: Standardization of Fluorescence Emmited by Rhodamine B. Ecotoxicology and Environmental Contamination, 8(1), 101–104. https://doi.org/10.5132/eec.2013.01.014

Issue

Vol. 8 No. 1 (2013)

Section

Original Articles

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Copyright (c) 2013 Ecotoxicology and Environmental Contamination

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